| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1001 | Bordetella avium | AATCGCCTTCCGTGTCGT | AAACGTAGGCCTTGCGCT | 59.35 | 59.97 | 170 | 68 |
100.00%
|
100.00%
|
| Primer 1002 | Bordetella avium | TGCCAAGGATGCCCTGAAG | ATGCCAGCAACGTGTCCA | 60.00 | 59.89 | 230 | 68 |
100.00%
|
100.00%
|
| Primer 1003 | Bordetella avium | ACGGTCACGCTCAAGCAT | GTCACGACGTATCTGCCGT | 59.66 | 59.86 | 152 | 68 |
100.00%
|
100.00%
|
| Primer 1004 | Bordetella avium | AGCGCAGGCAATCCATGT | ACAAACAAACGGGTGGCG | 60.04 | 59.20 | 198 | 68 |
100.00%
|
100.00%
|
| Primer 1005 | Bordetella avium | TGCGTGAAGGCAAACCCA | CAAGAAAGGGCATCACGCG | 60.12 | 59.86 | 205 | 68 |
100.00%
|
100.00%
|
| Primer 1006 | Bordetella avium | TGAAGATGCGCACGGTGT | AAACGCATGAGCAGGCCA | 59.97 | 60.28 | 163 | 68 |
100.00%
|
100.00%
|
| Primer 1007 | Bordetella avium | TGCGCACGGTGTTTGTTG | AAACGCATGAGCAGGCCA | 59.90 | 60.28 | 157 | 68 |
100.00%
|
100.00%
|
| Primer 1008 | Bordetella avium | ACGTCGAAGTCCGCAGTTT | AGGCGTCATGAACAGCGT | 59.93 | 59.66 | 170 | 68 |
100.00%
|
100.00%
|
| Primer 1009 | Bordetella avium | AACGTCGAAGTCCGCAGTT | AGGCGTCATGAACAGCGT | 59.93 | 59.66 | 171 | 68 |
100.00%
|
100.00%
|
| Primer 1010 | Bordetella avium | AGCCCTGTTCGACAAGGTG | AGGCGTCATGAACAGCGT | 59.93 | 59.66 | 283 | 68 |
100.00%
|
100.00%
|